PRODUCTION OF CELLULASE USING THERMOPHILLIC YEAST
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Abstract
Large quantities of byproducts generated during the processing of Agro-waste results in an economic and environmental problem due to their high volumes and
elimination costs. Corncob waste, banana peel, orange peel and pineapple are undervalued waste materials, unused source of energy that can serve as potential
source for cellulase production. This study was conducted to bioconvert agrowaste to cellulase using thermophilic yeast. The thermophillic organism of interest was isolated from hot region of a dumpsite in Benin City, plated using pour plate method and identified based on colonial and sugar fermentation characteristics. Purified isolates were screened for cellulase producing activity and the highest producer was used for further analyses. The standardized organism (Torulopsis bovina) was inoculated into each waste medium and incubated at 50
0C over the course of 10 days. pH, viable cell count and cellulase concentration was determined in two days interval. pH was determined using pH meter, viable cell
count was determined using pour plate method, while the cellulase concentration was determined using DNS method. The highest cellulase concentration was
obtained at day 8 with corncob waste medium; at a pH of 9.70±0.14 and cell count of 4.490±0.042 x 10
-4 cfu/ml. While the least was obtained with banana waste medium at day 8, with viable cell count of 0.730±0.028 cfu/ml and pH of 8.05±0.07 . Findings from this study suggest corncob as the best substrate for cellulase production using Torulopsis bovina. Hence, the recalcitrance nature of agrowaste in the environment
can be salvage through valorization, specifically into cellulase.
elimination costs. Corncob waste, banana peel, orange peel and pineapple are undervalued waste materials, unused source of energy that can serve as potential
source for cellulase production. This study was conducted to bioconvert agrowaste to cellulase using thermophilic yeast. The thermophillic organism of interest was isolated from hot region of a dumpsite in Benin City, plated using pour plate method and identified based on colonial and sugar fermentation characteristics. Purified isolates were screened for cellulase producing activity and the highest producer was used for further analyses. The standardized organism (Torulopsis bovina) was inoculated into each waste medium and incubated at 50
0C over the course of 10 days. pH, viable cell count and cellulase concentration was determined in two days interval. pH was determined using pH meter, viable cell
count was determined using pour plate method, while the cellulase concentration was determined using DNS method. The highest cellulase concentration was
obtained at day 8 with corncob waste medium; at a pH of 9.70±0.14 and cell count of 4.490±0.042 x 10
-4 cfu/ml. While the least was obtained with banana waste medium at day 8, with viable cell count of 0.730±0.028 cfu/ml and pH of 8.05±0.07 . Findings from this study suggest corncob as the best substrate for cellulase production using Torulopsis bovina. Hence, the recalcitrance nature of agrowaste in the environment
can be salvage through valorization, specifically into cellulase.
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