MICROBIAL ASSESSMENT OF INDOOR AIR OF MICROBIOLOGYLABORATORYIN UNIVERSITY OF BENIN, BENIN CITY
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Abstract
Microbiological quality assessment of indoor air study is one of the most vital investigationstodetermine the microbial indoor air contaminant. The information on the indoor microbial
concentrations of airborne bacteria and fungi is necessary both to estimate the health hazardandto create standards for indoor air quality control. Standard bacteriological methods were usedtodetermine the total bacterial count of the air samples. The bacterial isolates were characterizedand identified using morphological and biochemical methods. The percentage distributionandfrequency of the isolates were evaluated using statistical method. The results obtained fromthisstudy showed that laboratory one had the microbial load of 8.04x10
4 ± 6.0 Cfu/m3 whilelaboratory two had the microbial load of 6.85x10
4± 0.5 Cfu/m3
. Using standard morphological
and cultural characterization, the isolates obtained in this study were Staphylococcus aureus, Micrococcus sp, Bacillus subtilis, Streptococcus and Escherichia coli. The Grampositivebacteria were all susceptible to gentamycin and augmentin while the Gramnegative showedresistance to these antibiotics. However the isolates were all susceptible to ofloxacin. Theselected sampling laboratories of Faculty of Life Sciences, University of Beninwerecontaminated with bacteria. Thus, attention must be given to control those environmental factorswhich favor the growth and multiplication of microorganisms in indoor environment of theschool to safeguard health of users and workers, and it is vital to control visitors and studentsinand out the laboratories.
concentrations of airborne bacteria and fungi is necessary both to estimate the health hazardandto create standards for indoor air quality control. Standard bacteriological methods were usedtodetermine the total bacterial count of the air samples. The bacterial isolates were characterizedand identified using morphological and biochemical methods. The percentage distributionandfrequency of the isolates were evaluated using statistical method. The results obtained fromthisstudy showed that laboratory one had the microbial load of 8.04x10
4 ± 6.0 Cfu/m3 whilelaboratory two had the microbial load of 6.85x10
4± 0.5 Cfu/m3
. Using standard morphological
and cultural characterization, the isolates obtained in this study were Staphylococcus aureus, Micrococcus sp, Bacillus subtilis, Streptococcus and Escherichia coli. The Grampositivebacteria were all susceptible to gentamycin and augmentin while the Gramnegative showedresistance to these antibiotics. However the isolates were all susceptible to ofloxacin. Theselected sampling laboratories of Faculty of Life Sciences, University of Beninwerecontaminated with bacteria. Thus, attention must be given to control those environmental factorswhich favor the growth and multiplication of microorganisms in indoor environment of theschool to safeguard health of users and workers, and it is vital to control visitors and studentsinand out the laboratories.
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