EFFECT OF SALBUTAMOL

Salbutamol, montelukast, and prednisolone are commonly prescribed for respiratory disorders. While their therapeutic effects on the airways are well documented, their impact on pulmonary oxidative stress and antioxidant defense mechanisms remains less understood. This study investigated the effects of these drugs and their combinations on oxidative stress biomarkers and total protein concentration in lung tissue. Experimental animals were assigned to eight groups (n = 4): negative control, positive control, salbutamol, montelukast, prednisolone, salbutamol/prednisolone, salbutamol/montelukast, and prednisolone/montelukast. Lung tissue homogenates were analyzed for total protein concentration, antioxidant enzyme activitiesincluding superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx)-and oxidative markers, specifically hydrogen peroxide (H2O2) and nitric oxide (NO). Data were expressed as mean ±SEM and analyzed by one-way ANOVA with significance set at p < 0.05. Total protein concentration and catalase activity were not significantly altered in any treatment group compared with the negative control (p > 0.05). Prednisolone treatment significantly increased SOD activity relative to the negative control (p < 0.05), whereas GPx activity was elevated only in the positive control group. Montelukast significantly increased H202 levels compared with the negative control, but H202 levels in the salbutamol, montelukast, and prednisolone groups were significantly lower than the positive control. Nitric oxide concentration decreased in the positive control and prednisolone groups relative to the negative control, while montelukast treatment caused a significant increase in NO compared with the positive control (p < 0.05).