ISOLATION, CHARACTERIZATION AND HEMOLYSIN PRODUCTION OF BACTERIA ISOLATES FROM PALMS OF UNDERGRADUATE STUDENTS IN UNIVERSITY OF BENIN, BENIN CITY
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Abstract
Human hands usually harbor microorganisms both as part of normal flora and
microorganisms contacted from the environment. A prospective study was carried out in the University of Benin, Benin City, Nigeria. This study was aimed at identifying the types of bacteria associated with hands of undergraduate students in the University of Benin and to isolate and determine the hemolysin production of bacteria isolates present on the hands of the students. A total of 6 samples were obtained. The samples were analyzed using cultural, morphological and biochemical characteristics (citrate, indole, oxidase, catalase, motility, triple sugar ion and coagulase). The bacteria isolates recovered were S. aureus 3(25%), C. jeikeium 1(8.33), S. epidermidis 4(33.33), S. hominids 2(16.67) and Escherichia coli 2(16.67). All isolates of S. aureus, E. coli and S. hominis showed complete hemolysis (Beta hemolysis) while S. epidemidis and C. jeikeium were non hemolytic (Gamma hemolysis). All Gram positive isolates were resistant to amoxicillin, rifampin and septrin. Gram negative bacteria isolates were highly resistant to nalixidic acid, gentamicin, ciprofloxacin and ampicillin
microorganisms contacted from the environment. A prospective study was carried out in the University of Benin, Benin City, Nigeria. This study was aimed at identifying the types of bacteria associated with hands of undergraduate students in the University of Benin and to isolate and determine the hemolysin production of bacteria isolates present on the hands of the students. A total of 6 samples were obtained. The samples were analyzed using cultural, morphological and biochemical characteristics (citrate, indole, oxidase, catalase, motility, triple sugar ion and coagulase). The bacteria isolates recovered were S. aureus 3(25%), C. jeikeium 1(8.33), S. epidermidis 4(33.33), S. hominids 2(16.67) and Escherichia coli 2(16.67). All isolates of S. aureus, E. coli and S. hominis showed complete hemolysis (Beta hemolysis) while S. epidemidis and C. jeikeium were non hemolytic (Gamma hemolysis). All Gram positive isolates were resistant to amoxicillin, rifampin and septrin. Gram negative bacteria isolates were highly resistant to nalixidic acid, gentamicin, ciprofloxacin and ampicillin
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