DR. O. A. OLOGBOSERE

Study was carried out to investigate bacteria in urine contaminated soils within University of Benin Ugbowo campus. Five soil samples were collected and analyzed using soil serial dilution and pour plating techniques. Nutrient agar was used for bacteria isolation and subculture. Urine contaminated soil samples were collected from Hall three, Life science complex and Library extension while control soil samples were collected from Hall two car park and Hall three. Bacteria isolated from these soil samples and their prevalence include Bacillus subtilis (80%), Staphylococcus aureus (40%), Micrococcus luteus (60%), Escherichia coli (20%), Klebsiella pneumonia (40%) and Pseudomonas aeruginosa (60%). Hall three control soil had the highest bacteria count while Life science complex had the lowest bacteria count with the range of 5.5 × 10³ Cfu/ml and 7 × 10³ Cfu/ml. Various biochemical test were carried out which include oxidase test, citrate test, indole test, TSIA test, to identify isolates based on their reaction to these test. Antibactrial susceptibility test was also carried out. Antibiotics used include Gentamycin, Colistin, Carbenicillin, Ciprofloxacin and Tetracyclin. Urine contaminated soils are threats to the public’s health since they can be used as routes for the spread of infection.