Catechin Mercury chloride Heavy metal toxicity Cerebellar disorders Oxidative stress Neuroinflammation Purkinje cells Neurotoxicity Apoptosis

Reports indicate that cerebellar disorders are induced by exposure to heavy metals, such as Mercury, via oxidative stress, neuroinflammation, and Purkinje cell apoptosis, thus disrupting motor coordination and cognitive processing. Catechin (CA), a flavonoid in green tea, exhibits antioxidant, anti-tumor, and anti-inflammatory effects, making it a potential therapeutic agent against heavy metal toxicity. This study investigated the activity of catechin in the cerebellum of Wistar rats exposed to Mercury chloride. Sixty-four Wistar rats were randomly assigned into eight groups (n=8), and treated for twenty-eight days, as follows; A (control), B (5 mg/kg body weight [bw] of mercury chloride [HgCl2]), C (10 mg/kg bw of CA and HgCl2), D (20 mg/kg bw of CA and HgCl2), E (200 mg/kg bw of Vitamin E and HgCl2), F (10 mg/kg bw of Catechin only), G (20 mg/kg bw of Catechin only) and H (200 mg/kg bw of Vitamin E only). Thereafter, weights, neurobehavioral activities, mercury concentration, antioxidant enzymes activity, lipid peroxidation, and histology of the cerebellum were assessed. In-silico studies were utilized to investigate multi-targeted protective potential of catechin, by assessing its interactions with key mediators of inflammation, oxidative stress, and apoptosis. Findings showed that HgCl₂ treatment significantly decreased (p<0.05) body weight and antioxidant enzymes, while significantly increasing (p<0.05) lipid peroxidation, mercury concentration, and neurobehavioral deficits, with cerebellar histology revealing Purkinje cell alterations, vacuolations, and pyknotic nuclei. Conversely, groups pre-treated with CA showed an attenuation of the HgCl2-induced adverse effects. In-silico results showed catechin bound more strongly to Nrf2, TNF-α, IL-6, NF-ĸB, and Caspase-3 than amantadine and riluzole, highlighting its multi-targeted antioxidant, anti-inflammatory, and anti-apoptotic potential.
This study highlights catechin as a safe, natural therapeutic for HgCl2-induced cerebellar disorder, offering a promising alternative to conventional drugs with fewer side effects Furtherstudies exploring the translational application of catechin in clinical trials are recommended