ROPONIN-I LEVEL IN HEAT-EXPOSED FEMALE SPRAGUE- DAWLEY RATS
Department
Year of Publication
upload
Publication Type
Abstract
roponin I (TnI) is a crucial component of the troponin complex in striated muscle, playing a central role in regulating contraction and relaxation through calcium interaction. Heat stress refers to the overall response of the human body to the combined effects of environmental factors and temperature. It indicates how much heat the body is exposed to within its thermal surroundings. The justification for studying troponin I levels in heat-exposed Sprague-Dawley female rats is grounded in the need to understand cardiac responses to hyperthermia. Elevated troponin I levels can indicate myocardial injury, which is critical during heat stress conditions. The study aimed to explore the connection between heat exposure and myocardial damage by
examining variations in cardiac troponin I (cTnI) levels in heat-exposed Sprague-Dawley female rats. The rats were randomly divided into four groups (1, 2, 3 and 4) for a period of eight weeks. The rats were exposed to heat for 1 to 2 hours daily at a temperature of 38°C to 40°C. Group 1 served as the control group which were not exposed to heat. Group 2 was exposed to heat for 14 days and group 3 and 4 were exposed to heat for 28 days and 42 days respectively. At the end of the experiment, each animal was first anaesthetized using chloroform vapour, followed by a dissection procedure to harvest the heart tissue. After harvesting the heart tissue, it was minced into small pieces and homogenised using phosphate buffer solution, thereafter spun and the supernatant was collected and sent for biochemical analysis. Statistical analysis was done using graph pad prism version 10.4. Results were presented as mean ± standard error of mean. One Way Analysis of Variance (ANOVA) was used to compare the means of tests and control value while post hoc test was done using Dunnett's multiple comparison test and a P-value of less than 0.05 was considered statistically significant. The result gotten from this research shows that mild heat exposure may not induce noticeable cardiac stress. In conclusion, long periods of high temperature exposure may indicate possible myocardial alteration, so heat exposure should be kept within reasonable limits
examining variations in cardiac troponin I (cTnI) levels in heat-exposed Sprague-Dawley female rats. The rats were randomly divided into four groups (1, 2, 3 and 4) for a period of eight weeks. The rats were exposed to heat for 1 to 2 hours daily at a temperature of 38°C to 40°C. Group 1 served as the control group which were not exposed to heat. Group 2 was exposed to heat for 14 days and group 3 and 4 were exposed to heat for 28 days and 42 days respectively. At the end of the experiment, each animal was first anaesthetized using chloroform vapour, followed by a dissection procedure to harvest the heart tissue. After harvesting the heart tissue, it was minced into small pieces and homogenised using phosphate buffer solution, thereafter spun and the supernatant was collected and sent for biochemical analysis. Statistical analysis was done using graph pad prism version 10.4. Results were presented as mean ± standard error of mean. One Way Analysis of Variance (ANOVA) was used to compare the means of tests and control value while post hoc test was done using Dunnett's multiple comparison test and a P-value of less than 0.05 was considered statistically significant. The result gotten from this research shows that mild heat exposure may not induce noticeable cardiac stress. In conclusion, long periods of high temperature exposure may indicate possible myocardial alteration, so heat exposure should be kept within reasonable limits
Supervisor(s)
co-supervisor