ISOLATION, IDENTIFICATION AND ANTIBIOGRAM OF BACTERIA FROM DUNG OF LOCAL CATTLE
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Abstract
Cattle dung contains a lot of potentially pathogenic bacteria. Data on bacteria from local cattle
(muturu) unlike its fulani relative is however scarce. This research was carried out to analyze the
bacterial microflora of cattle muturu dung. A total of four stool samples received in our
Laboratory were analyzed. A ten-fold serial dilution was carried out on all samples. Briefly, one
(1) gram each of stool sample was weighed onto test tubes containing 9ml of sterile distilled
water to make stock solutions. The tubes were homogenized after which 1ml was inoculated onto
petri dish containing solidified agar and rocked for even distribution. Agar plates were incubated
at 37°∁ for 24hours. Bacterial counts were carried out on each of them. Identification of bacteria
isolates were carried out using standard guidelines such as morphological (shape, size, arrangement, staining property, motility and spore formation) colonial (texture, elevation, margin
and colour) and biochemical (catalase, oxidase, citrate utilization, urease and indole production)
characteristics. Bacterial isolates recovered from cattle dung incude: Eschericia coli, Salmonella
sp.,Staphylococcus sp., Enterococcus sp., Shigella sp., Bacillus sp. and Streptococcus sp. The
antibacterial resistance pattern of bacterial isolates to routinely used antibiotics (azithromycin, levofloxacin, erythromycin, pefloxacin, gentamycin, ampicillin-cloxacillin, cefuroxime, amoxiclin, ceftriaxone and ciprofloxacin) was carried out using the Disc diffusion method. The
total heterotrouphic bacterial counts ranged from 1.5×10
3cfu/g – 9.8 ×10
5 cfu/g. Bacterial
isolates showed varying resistance to the antibacterial agents used in this study. Both isolates of
Eschericia coli were resistant to azithromycin and levofloxacin. Bacillus specie was resistant to
erythromycin and pefloxacin while Salmonella sp. showed resistance to gentamycin. The
presence of antibiotic resistance bacteria in local cattle dug could be of grave public health
concern
(muturu) unlike its fulani relative is however scarce. This research was carried out to analyze the
bacterial microflora of cattle muturu dung. A total of four stool samples received in our
Laboratory were analyzed. A ten-fold serial dilution was carried out on all samples. Briefly, one
(1) gram each of stool sample was weighed onto test tubes containing 9ml of sterile distilled
water to make stock solutions. The tubes were homogenized after which 1ml was inoculated onto
petri dish containing solidified agar and rocked for even distribution. Agar plates were incubated
at 37°∁ for 24hours. Bacterial counts were carried out on each of them. Identification of bacteria
isolates were carried out using standard guidelines such as morphological (shape, size, arrangement, staining property, motility and spore formation) colonial (texture, elevation, margin
and colour) and biochemical (catalase, oxidase, citrate utilization, urease and indole production)
characteristics. Bacterial isolates recovered from cattle dung incude: Eschericia coli, Salmonella
sp.,Staphylococcus sp., Enterococcus sp., Shigella sp., Bacillus sp. and Streptococcus sp. The
antibacterial resistance pattern of bacterial isolates to routinely used antibiotics (azithromycin, levofloxacin, erythromycin, pefloxacin, gentamycin, ampicillin-cloxacillin, cefuroxime, amoxiclin, ceftriaxone and ciprofloxacin) was carried out using the Disc diffusion method. The
total heterotrouphic bacterial counts ranged from 1.5×10
3cfu/g – 9.8 ×10
5 cfu/g. Bacterial
isolates showed varying resistance to the antibacterial agents used in this study. Both isolates of
Eschericia coli were resistant to azithromycin and levofloxacin. Bacillus specie was resistant to
erythromycin and pefloxacin while Salmonella sp. showed resistance to gentamycin. The
presence of antibiotic resistance bacteria in local cattle dug could be of grave public health
concern
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