UTILIZATION OF SELECTED WASTE EXTRACTS ON THE VEGETATIVE GROWTH OF Marasmiellus inoderma (Berk.) SINGER
Faculty
Department
Year of Publication
Keyword
upload
Publication Type
Abstract
Mushrooms are good degraders of wastes and plant litters. Marasmiellus inoderma is an edible and medicinal mushroom that usually grows on leaf litters and wood wastes. To test for the suitability of plant wastes on the vegetative growth of the mushroom, extracts from selected wastes were used to prepare media for its growth in the laboratory. Six plant wastes were
selected for this study. They include Oil palm fruit fiber (OPFF), Banana leaves, Terminalia catappa leaves (TC), paper wastes , cotton wastes and melon husk wastes. The extracts of each these wastes were prepared by weighing 100g of the waste into 1L of water and boiling for 15 minutes. The extracts were amended with Dextrose sugar and PDA and then inoculated a 10mm
agar plug of a 7day old culture. The results obtained indicated that the extract from cotton waste (75.75 ± 12.71 mm) and cotton amended with PDA gave the highest mycelia extension (74.33 ± 1.61 mm) followed by Oil palm fruit fiber extract amended with PDA (73.5 ± 3.04 mm) and the least extension was observed in the extract of waste paper amended with PDA (39.33 ± 29.17 mm). The extract of cotton amended with PDA gave the highest biomass yield after 12 days of inoculation. The pure extract of Banana leaves compared well with PDA in the mycelia extension of the mushroom while Melon amended with PDA compared favorably with PDA in biomass yield of the mycelium. The density and morphology of the mushroom on the extracts
differed considerably. While the mycelia growth and form was dense in pure banana and cotton extracts, it was slightly dense in the pure extracts of OPFF and Terminalia catappa leaf and very dense in melon husk and cotton waste extracts when both were amended with PDA. This study shows that the fungus can be cultivated on these wastes and can therefore be used to reduce the waste and convert these wastes into food. These substrates can also be useful in generating the mycelia of the fungus Marasmiellus inoderma to produce other value-added products
selected for this study. They include Oil palm fruit fiber (OPFF), Banana leaves, Terminalia catappa leaves (TC), paper wastes , cotton wastes and melon husk wastes. The extracts of each these wastes were prepared by weighing 100g of the waste into 1L of water and boiling for 15 minutes. The extracts were amended with Dextrose sugar and PDA and then inoculated a 10mm
agar plug of a 7day old culture. The results obtained indicated that the extract from cotton waste (75.75 ± 12.71 mm) and cotton amended with PDA gave the highest mycelia extension (74.33 ± 1.61 mm) followed by Oil palm fruit fiber extract amended with PDA (73.5 ± 3.04 mm) and the least extension was observed in the extract of waste paper amended with PDA (39.33 ± 29.17 mm). The extract of cotton amended with PDA gave the highest biomass yield after 12 days of inoculation. The pure extract of Banana leaves compared well with PDA in the mycelia extension of the mushroom while Melon amended with PDA compared favorably with PDA in biomass yield of the mycelium. The density and morphology of the mushroom on the extracts
differed considerably. While the mycelia growth and form was dense in pure banana and cotton extracts, it was slightly dense in the pure extracts of OPFF and Terminalia catappa leaf and very dense in melon husk and cotton waste extracts when both were amended with PDA. This study shows that the fungus can be cultivated on these wastes and can therefore be used to reduce the waste and convert these wastes into food. These substrates can also be useful in generating the mycelia of the fungus Marasmiellus inoderma to produce other value-added products
Supervisor(s)
co-supervisor