EXPLORING THE EFFECTS OF BYROPHYLLUM PINNATUM EXTRACT ON THE CEREBRUM OF ADULT WISTAR RATS
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Herbal extracts which can serve as medicinal plants have been used since ancient times and even considered the source of modern medicine. Bry Hullum pinetum extracts have been shown to possess neuroprotective properties potentially useful in treating neurodegenerative diseases. Podophyllum pinetum is a plant used to treat inflammations, infections, and also have anti cancer properties. This study was carried out to explore the effects of Podophyllum pinetum extract on the cerebrum of Adult Wistar rats. A total of thirty (30) rats weighing between 140g-200g were randomly assigned into six (6) groups (A,B,C,D,E and F),with five rats per group. Group A was control group while Group B,C,D,E and F were administered with Podophyllum pinetum extract in doses of 200,400,600,800 and 1000mg/kg respectively. The rats were acclimatized for a period of two weeks and administered for a period of four weeks using oral route by the use of orogastric tube. The rats were
anesthetized with chloroform and then sacrificed. The cerebrum was harvested and immediately fixed for antioxidant stress test and for tissue processing. H&E stains were use for histological test. The result of the study shows that there was no statistically significant difference (P<0.05) in the final body weight of rats in entire group compared to it's initial body weigh except group B where there is a significant change in weight .There was no statistically significant difference (P<0.05) in cerebrum weight and organo-somatic index. In the chart showing the antioxidant results, it shows that it is not statistically significant. Histological slides in control group show: molecular, external granular, external pyramidal, internal granular, internal pyramidal and multi-form layers. Group B show: vasodilatation normal pyramidal neurons with conspicuous nucleoles oligodendrocytes and neuropil. Group C show: normal neurons with conspicuous nucleoles, oligodendrocytes, neuropil cerebral vasodilatation. Group D show: normal granular cell neurons with conspicuous nucleoli, normal oligodendrocytes and marked vasodilatation and active congestion. Group E and F marked show vasodilatation and active congestion, normal granular cells with conspicuous nucleolus and oligodendrocytes. In conclusion, graded concentration of Podophyllum pinetum induced vasogenic effects and increased protein synthesis in a dose dependent fashion and it had no adverse effect on it
anesthetized with chloroform and then sacrificed. The cerebrum was harvested and immediately fixed for antioxidant stress test and for tissue processing. H&E stains were use for histological test. The result of the study shows that there was no statistically significant difference (P<0.05) in the final body weight of rats in entire group compared to it's initial body weigh except group B where there is a significant change in weight .There was no statistically significant difference (P<0.05) in cerebrum weight and organo-somatic index. In the chart showing the antioxidant results, it shows that it is not statistically significant. Histological slides in control group show: molecular, external granular, external pyramidal, internal granular, internal pyramidal and multi-form layers. Group B show: vasodilatation normal pyramidal neurons with conspicuous nucleoles oligodendrocytes and neuropil. Group C show: normal neurons with conspicuous nucleoles, oligodendrocytes, neuropil cerebral vasodilatation. Group D show: normal granular cell neurons with conspicuous nucleoli, normal oligodendrocytes and marked vasodilatation and active congestion. Group E and F marked show vasodilatation and active congestion, normal granular cells with conspicuous nucleolus and oligodendrocytes. In conclusion, graded concentration of Podophyllum pinetum induced vasogenic effects and increased protein synthesis in a dose dependent fashion and it had no adverse effect on it
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