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Abstract
Excessive prostaglandin production had been reported to lead to uterine contraction and premature cervical changes contributing to preterm birth. The aim of this study is to determine the effect of negro pepper on the prostaglandin level during the gestation period of wistar rat. Sixteen (16) female wistar rats weighing 140 to 160 were purchased from the animal house of the Department of Anatomy, University of Benin. The rats were acclimatized for two weeks to the laboratory condition with free access to food and water.
The dry fruits after being grind were soaked with n- hexane using maceration method for 24 hrs and were filtered to obtain the filtrate which was evapourated to obtain the oil. The female rats were mated with male rate in the ratio of 2:1. The rats were grouped into 4 groups (A, B, C, D) in two phases (Germinal and Embryonic Phase) and administered negro pepper extract orally at the doses of 0.25ml/Kg, 0.5ml/Kg and 1ml/Kg respectively daily for 7 days. The rats were sacrificed using chloroforms ane thesia, the uterus was collect from the animal and homogenated with buffer solution and then centrifuged to collect the Supernatant which was used for the biochemical analysis.
The result were statistically analyzed, results obtained were expressed as mean ±SEM (Standard Error of Mean). Differences among the means were determined by one way analysis of variance (ANOVA). Values were considered statistically significant if P value is less than 0.05 (P<0.05). Results: there was a significant increase in PGE2 level at 0.5ml/kg compared with control, though there was no significant differences in PGE2 at 0.25ml/kg compared with control in the embryonic phase and there was a significant decrease in PGE2 at 0.25ml/kg and 0.5ml/g in the Germinal phase compared with control though there was no significant difference in 1.0ml/kg compared to control. In conclusion, Negro Pepper Extract at 0.5ml/kg significantly increased PGE2 level at the embryonic phase but significantly decreased PGE2 in 0.25ml/kg and 0.5ml/kg in the germinal phase compared to control showing that administration of negro pepper extract during pregnancy maybe detrimental to the pregnancy.
The dry fruits after being grind were soaked with n- hexane using maceration method for 24 hrs and were filtered to obtain the filtrate which was evapourated to obtain the oil. The female rats were mated with male rate in the ratio of 2:1. The rats were grouped into 4 groups (A, B, C, D) in two phases (Germinal and Embryonic Phase) and administered negro pepper extract orally at the doses of 0.25ml/Kg, 0.5ml/Kg and 1ml/Kg respectively daily for 7 days. The rats were sacrificed using chloroforms ane thesia, the uterus was collect from the animal and homogenated with buffer solution and then centrifuged to collect the Supernatant which was used for the biochemical analysis.
The result were statistically analyzed, results obtained were expressed as mean ±SEM (Standard Error of Mean). Differences among the means were determined by one way analysis of variance (ANOVA). Values were considered statistically significant if P value is less than 0.05 (P<0.05). Results: there was a significant increase in PGE2 level at 0.5ml/kg compared with control, though there was no significant differences in PGE2 at 0.25ml/kg compared with control in the embryonic phase and there was a significant decrease in PGE2 at 0.25ml/kg and 0.5ml/g in the Germinal phase compared with control though there was no significant difference in 1.0ml/kg compared to control. In conclusion, Negro Pepper Extract at 0.5ml/kg significantly increased PGE2 level at the embryonic phase but significantly decreased PGE2 in 0.25ml/kg and 0.5ml/kg in the germinal phase compared to control showing that administration of negro pepper extract during pregnancy maybe detrimental to the pregnancy.
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