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Abstract
Water is a vital resource for all known living organisms, as there is no life without it. The Earth’s surface is 71% water and fresh water comprises 3% of the total amount water on the Earth’s surface. The aim of the present study was to determine the physicochemical parameters and isolation of Fungi from reservoir Water in different communities in Benin City. Water samples were collected from Adolor, BDPA, Ekosodin, Isihor, Osasogie, Uniben and Uwasota all in Benin City, Edo State. The samples were then taken to the laboratory and cultured on potato dextrose agar using the pour plate technique. The pure culture of fungal isolates were identified phenotypically. The isolates antifungal susceptibility profiles were determined
The results for physiochemical analysis of the reservoir water sample showed that the pH values were within the range of 7.59 – 6.63, electrical Conductivity ranged from 13 - 159 µS/cm. The temperature range gotten from this study is 25.3 – 29.4 oC. Total suspended solid varied from 0.59 – 0.98 g/l. Biochemical oxygen demand ranged from 0.02 – 0.07. The alkalinity ranged from 0.12 to 0.5 g/l. Manganese was present in the ranged from 0.01 to 0.15 mg/l. The total fungal count was shown a ranged of 0.025 x 103 - 2.3 x 103 cfu/ml. The highest fungal count was found in Ekosodin, having a value of 2.3 x 103 cfu/ml while the lowest was from Osasogie, value of 0.025 x 103 cfu/ml. The identified fungal isolates from the various samples of water were Penicilium chrysogenum, Yeast, Rhizopus stolonifera, Aspergillus flavus and Aspergilus niger. The isolate with the lowest percentage distribution was Penicilium chrysogenum, asides Penicilium chrysogenum the rest of the isolates were found in all the sampled locations. The pathogenicity result showed all isolates were positive to Gamma Haemolysis and Lipase production. All isolates showed susceptibility to Nystatin but showed varying resistance to Vericonazole with penicillin showing the highest susceptibility. Recent discoveries on fungi requiring special attention include the presence of opportunistic and emerging pathogens in water sources.
The results for physiochemical analysis of the reservoir water sample showed that the pH values were within the range of 7.59 – 6.63, electrical Conductivity ranged from 13 - 159 µS/cm. The temperature range gotten from this study is 25.3 – 29.4 oC. Total suspended solid varied from 0.59 – 0.98 g/l. Biochemical oxygen demand ranged from 0.02 – 0.07. The alkalinity ranged from 0.12 to 0.5 g/l. Manganese was present in the ranged from 0.01 to 0.15 mg/l. The total fungal count was shown a ranged of 0.025 x 103 - 2.3 x 103 cfu/ml. The highest fungal count was found in Ekosodin, having a value of 2.3 x 103 cfu/ml while the lowest was from Osasogie, value of 0.025 x 103 cfu/ml. The identified fungal isolates from the various samples of water were Penicilium chrysogenum, Yeast, Rhizopus stolonifera, Aspergillus flavus and Aspergilus niger. The isolate with the lowest percentage distribution was Penicilium chrysogenum, asides Penicilium chrysogenum the rest of the isolates were found in all the sampled locations. The pathogenicity result showed all isolates were positive to Gamma Haemolysis and Lipase production. All isolates showed susceptibility to Nystatin but showed varying resistance to Vericonazole with penicillin showing the highest susceptibility. Recent discoveries on fungi requiring special attention include the presence of opportunistic and emerging pathogens in water sources.
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