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A common industrial chemical, bisphenol-A (BPA) is connected to oxidative stress, memory loss, learning impairment, reduced cholinergic function, and neuronal degeneration. BPA is also utilized in
the manufacturing of polycarbonate, epoxy resins, and plastics. Ascorbic acid, another name for vitamin C, is a necessary substance that is involved in several biological activities. It has been proposed as a potential therapeutic intervention for oxidative stress because of its strong antioxidant properties, which shield the body from oxidative damage brought on by free radicals. On the other hand, opinions about the protective role of vitamin C in bisphenol-A-induced toxicity are divided. This research looked at the neuroprotective effects of vitamin C in the context of toxicity caused by bisphenol-A in Drosophila melanogaster. Three to five days ago, flies were divided into groups. Group 2 received a diet containing 1 mM of bisphenol-A (BPA), whereas Group 1 acted as the control group. 200 mM of vitamin C was given to Group 3 by food, whereas Group 4 received 200 mM of vitamin C plus 1 mM of BPA through food. For six (6) days, the flies were kept on these treatments at room temperature. To evaluate locomotor performance, an open field research and negative geotaxis were conducted (climbing activity and exploratory movement). Additionally, a 15-day survival research was conducted to look at the effects of vitamin C and bisphenol A on fly survival rates. After the experiment was over, the flies were homogenized, and the supernatants were used to measure the activities of glutathione S-transferase (GST), acetylcholinesterase (AChE), superoxide dismutase (SOD), catalase (CAT), malonaldehyde
(MDA), and catalase-catalase. The survival rate, motility, and climbing activity (negative geotaxis) of flies treated with BPA were all significantly reduced. Additionally, the activities of AChE, MDA, Catalase, SOD, and BPA-treated flies were reduced. Vitamin C was able to considerably raise the flies' survival rate, motility, and climbing activity throughout the co-treatment procedure. It also lessened the effects of the BPA increase on AChE activity and MDA levels in these flies. Furthermore, vitamin C inhibited and BPA-induced redu tion in GST activity was observed.
the manufacturing of polycarbonate, epoxy resins, and plastics. Ascorbic acid, another name for vitamin C, is a necessary substance that is involved in several biological activities. It has been proposed as a potential therapeutic intervention for oxidative stress because of its strong antioxidant properties, which shield the body from oxidative damage brought on by free radicals. On the other hand, opinions about the protective role of vitamin C in bisphenol-A-induced toxicity are divided. This research looked at the neuroprotective effects of vitamin C in the context of toxicity caused by bisphenol-A in Drosophila melanogaster. Three to five days ago, flies were divided into groups. Group 2 received a diet containing 1 mM of bisphenol-A (BPA), whereas Group 1 acted as the control group. 200 mM of vitamin C was given to Group 3 by food, whereas Group 4 received 200 mM of vitamin C plus 1 mM of BPA through food. For six (6) days, the flies were kept on these treatments at room temperature. To evaluate locomotor performance, an open field research and negative geotaxis were conducted (climbing activity and exploratory movement). Additionally, a 15-day survival research was conducted to look at the effects of vitamin C and bisphenol A on fly survival rates. After the experiment was over, the flies were homogenized, and the supernatants were used to measure the activities of glutathione S-transferase (GST), acetylcholinesterase (AChE), superoxide dismutase (SOD), catalase (CAT), malonaldehyde
(MDA), and catalase-catalase. The survival rate, motility, and climbing activity (negative geotaxis) of flies treated with BPA were all significantly reduced. Additionally, the activities of AChE, MDA, Catalase, SOD, and BPA-treated flies were reduced. Vitamin C was able to considerably raise the flies' survival rate, motility, and climbing activity throughout the co-treatment procedure. It also lessened the effects of the BPA increase on AChE activity and MDA levels in these flies. Furthermore, vitamin C inhibited and BPA-induced redu tion in GST activity was observed.
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