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Abstract
Phyllanthus amarus(UBH-P406) is a small annual plant widely used in traditional medicine in many parts of the world. It's been investigated for various potential health benefits, including antiviral, hepatoprotective, and anticancer properties. Piper guineense(UBH-P351) which is commonly known as Ashanti pepper or West African black pepper, is a West African spice with a
pungent flavor. It contains various bioactive compounds, including alkaloids and amides. Some studies suggest that Piper guineense may have antioxidant, anti-inflammatory, and anticancer properties.DMH (1,2-dimethylhydrazine): DMH is a chemical compound that is a potent colon carcinogen, commonly used in experimental studies to induce colon cancer in laboratory animals. It undergoes metabolic activation in the body, leading to the formation of reactive metabolites that can damage DNA and promote tumor development in the colon. The aim and objective of this study is to evaluate the therapeutic effects of aqueous extract of Phyllanthus amarus leaves mixed with Piper guineense leaves on 1,2 Dimethylhydrazine-induced colon cancer in Swiss Albino rat. In this present study, the body weight of the animals weighed from 150g-256g. The Rats were then separated into groups of 3 with 6 animals in each group, marked at different positions for easy identification of the rats and placed into plastic cages which had granular cellulose bedding. The rats were fed with growers' mash in regular pellets, they were also given tap water with constant light. Randomization was used with graphpad.com in respect to ARRIVE guideline. The groups the rats were divided into group 1 (control group), Group 2(20mg/kg of DMH bwt P. amarus and P. guinnense) Group 3(20mg/kg of DMH only). In the Liver, AST and ALT, control and DMH + P. amarus and P. guinnense were significantly different when compared to that of DMH only. In Kidney, Urea and Creatinine, control and DMH only were significantly different from DMH + P. amarus and P. guineense group. In electrolyte, Na+, K+, HCO3 and Cl, no significant difference was observed when control was compared to plant treated and DMH only groups. This suggest that aqueous extract + P. amarus and P. guineense have anti-oxidant, anti-cancer, and hepatorenal protective potentials.
pungent flavor. It contains various bioactive compounds, including alkaloids and amides. Some studies suggest that Piper guineense may have antioxidant, anti-inflammatory, and anticancer properties.DMH (1,2-dimethylhydrazine): DMH is a chemical compound that is a potent colon carcinogen, commonly used in experimental studies to induce colon cancer in laboratory animals. It undergoes metabolic activation in the body, leading to the formation of reactive metabolites that can damage DNA and promote tumor development in the colon. The aim and objective of this study is to evaluate the therapeutic effects of aqueous extract of Phyllanthus amarus leaves mixed with Piper guineense leaves on 1,2 Dimethylhydrazine-induced colon cancer in Swiss Albino rat. In this present study, the body weight of the animals weighed from 150g-256g. The Rats were then separated into groups of 3 with 6 animals in each group, marked at different positions for easy identification of the rats and placed into plastic cages which had granular cellulose bedding. The rats were fed with growers' mash in regular pellets, they were also given tap water with constant light. Randomization was used with graphpad.com in respect to ARRIVE guideline. The groups the rats were divided into group 1 (control group), Group 2(20mg/kg of DMH bwt P. amarus and P. guinnense) Group 3(20mg/kg of DMH only). In the Liver, AST and ALT, control and DMH + P. amarus and P. guinnense were significantly different when compared to that of DMH only. In Kidney, Urea and Creatinine, control and DMH only were significantly different from DMH + P. amarus and P. guineense group. In electrolyte, Na+, K+, HCO3 and Cl, no significant difference was observed when control was compared to plant treated and DMH only groups. This suggest that aqueous extract + P. amarus and P. guineense have anti-oxidant, anti-cancer, and hepatorenal protective potentials.
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