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Abstract
Isotretinoin (Accutane), a retinoid commonly prescribed for severe acne, has been shown to induce oxidative stress and organ toxicity during pregnancy, leading to developmental abnormalities in the fetus. The liver and kidneys are particularly prone to Accutane-induced damage due to their vital roles in detoxification and excretion. Previous studies has shown that quercetin scavenges free radicals and inhibits lipid peroxidation, while vitamin E stabilizes cell membranes and enhances antioxidant defense mechanisms. This study investigated the attenuating effects of quercetin, a natural flavonoid with antioxidant and anti-inflammatory properties, and vitamin E, a lipid-soluble antioxidant, on Accutane-induced liver and kidney toxicity during prenatal development. Pregnant Wistar rats were divided into four groups of five animals each: Control, Accutane only, Accutane + Vitamin E, and Accutane + Quercetin + Vitamin E. Administration was carried out from gestation day 14–21, and the littered fetuses
were sacrificed on postnatal day 30 for biochemical and histological analyses. Data was analysed using the SPSS statistical tool. Results showed that exposure to Accutane led to reduced birth weight and crown-rump length compared to other groups, while co-treatment with quercetin and vitamin E improved birth weight comparable to the control. Histological findings of the Accutane-only and Accutane + Vitamin E groups showed periportal inflammation in the liver while the Accutane-only group showed interstitial congestion and tubular swelling in the kidneys, both markedly reduced by co-treatment. In conclusion, the
combination of quercetin and vitamin E significantly attenuated Accutane-induced hepatorenal toxicity, highlighting their potential as protective antioxidants for maternal and fetal health
were sacrificed on postnatal day 30 for biochemical and histological analyses. Data was analysed using the SPSS statistical tool. Results showed that exposure to Accutane led to reduced birth weight and crown-rump length compared to other groups, while co-treatment with quercetin and vitamin E improved birth weight comparable to the control. Histological findings of the Accutane-only and Accutane + Vitamin E groups showed periportal inflammation in the liver while the Accutane-only group showed interstitial congestion and tubular swelling in the kidneys, both markedly reduced by co-treatment. In conclusion, the
combination of quercetin and vitamin E significantly attenuated Accutane-induced hepatorenal toxicity, highlighting their potential as protective antioxidants for maternal and fetal health
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