EFFECTS OF BISPHENOL-A AND SELENIUM ON SOME OXIDATIVE STRESS MARKERS IN MALE WISTAR RATS

Bisphenol-A (BPA) is an industrial chemical primarily used in the production of polycarbonate plastics and epoxy resins. Selenium (Se) is an essential trace element, vital for the health of humans and other living organisms. The aim of this study was to investigate the effects of Bisphenol-A (BPA) on some oxidative stress markers in adult male rats and evaluate the potential effect of Selenium (Se) in BPA-induced oxidative damage. A total of twenty (20) male Wistar rats weighing between 180g and 200g were purchased and kept in standard cages for two weeks to enable them acclimatize to their new environment. After acclimatization period, the twenty-adult male Wistar rats were divided into four (4) different groups A, B, C, D: control, BPA-only, Se-only, and BPA+Se. Group A served as control, Groups B D received 20mg/kg BPA, 2mg/kg Se, and both, respectively, for 54 days. Blood was collected and analyzed for oxidative stress parameters such as malondialdehyde (MDA), superoxide Distumates (SOD) and catalase (CAT). All statistical analyses were carried out using Graph Pad prism statistical software version 10.0. The data from all the groups were presented as Mean ± S.E.M (Standard Error of Mean), (n=5) in each group and analyze for statistical significance using one-way Analysis of Variance (ANOVA). Values were considered significant at P<0.05. The result shows that exposure to BPA resulted in significant (p<0.05) reduction in body weight. There was significant (p<0.05) increase in MDA levels in all groups compared with the control. On the other hand, SOD and CAT activities were significantly (p<0.05) reduced in all groups compared with the control, thereby indicating decreased antioxidant enzyme activities. In conclusion, these finding shows that Selenium (Se) supplementation did not mitigate the adverse effects of BPA and instead worsened oxidative stress, implying that Selenium (Se) may not provide protection against the harmful effect of Bisphenol-A (BPA) even at this dose but rather potentiated the effect of Bisphenol-A (BPA).